Detection of Cherry Leaf Roll Virus and Strawberry Latent Ring Spot Virus by One-Step RT-PCR
نویسنده
چکیده
Kumari S. (2009): Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RTPCR. Plant Protect. Sci., 45: 140–143. A one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of Cherry leaf roll virus (CLRV) and Strawberry latent ring spot virus (SLRSV). The protocol was used to test infected screen house plants and also plants from orchards and vineyards where the vector (Xiphinema diversicaudatum) of SLRSV was detected from the soil. The one-step RT-PCR protocol is rapid and sensitive and has the potential to be used for the diagnosis of CLRV and SLRSV in routine diagnostic laboratories.
منابع مشابه
Detection and Identification of Tomato Ring Spot Virus in Ornamental Plants in North Khorasan Province
In recent years, the symptoms of viral diseases such as dwarfism, mosaic, discoloration, necrosis, and circular spots have been prevalent in ornamental plants in parks, gardens, and streets in North Khorasan Province. Tomato ring spot virus (ToRSV) belongs to the Nepovirus genus of Secoviridae family. This virus is a major cause of the infection of ornamental plants in the world. In this study,...
متن کاملAn improved reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of two cherry flexiviruses in Prunus spp.
A one-step reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Cherry green ring mottle virus (CGRMV) and Cherry necrotic rusty mottle virus (CNRMV) in woody indicators and naturally infected Prunus spp. Viral RNA suitable for RT-PCR was obtained by a simple trapping method that did not require either extraction of double-stranded RNA (dsRNA) or total RNA,...
متن کاملAn Investigation of Apple Chlorotic Leaf Spot (ACLSV) and Tomato Ring Spot (ToRSV) in Some Iranian Pear Gardens
Background and Aims: In spite of a long tradition of fruit-tree growing in all provinces of Iran, information on pear (Pyrus communis) tree viruses is scant. In order to identify of the nature of the virus like symptoms and decline in pear trees, we carried out a survey for the occurrence of four important viruses. Materials and Methods: Leaf samples of pear showing virus like symptoms wer...
متن کاملSimultaneous Detection of Arabis Mosaic Virus, Cherry Leafroll Virus and Cucumber Mosaic Virus with Coamplification of Plant mRNA as Internal Control for Olive Certification Programs
Background and Aims: Certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. Reverse transcription-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certific...
متن کاملBiological and Serological Procedures to Detect Three Nepoviruses in Fruit Trees
P���� J., C���������� M., J���� M. (2004): Biological and serological procedures to detect three nepoviruses in fruit trees. Plant Protect. Sci., 40: 121–127. Cherry leaf roll virus (CLRV), Myrobalan latent ringspot virus (MLRSV) and Strawberry latent ringspot virus (SLRSV) were transferred by budding to woody trees, hybrid Ishtara, peach cv. GF 305 and cv. Lesiberian. Three buffers with antiox...
متن کامل